Journal: PLoS ONE

Article Title: Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix

doi: 10.1371/journal.pone.0107339

Figure Lengend Snippet: Immediately before and 3, 7 days after incubation, cell clots were harvested and confocal microscopy was performed to detect fluorescent immunostaining for COL1. Results showed that the expression of COL1 was absent or weak in two groups before incubation, but stronger in NPWT group at day 3 and 7 after incubation. Bars = 50 µm.

Article Snippet: Then they were blocked with goat serum, and incubated with the specific rat primary antibodies against collagen type 1 (COL1) (1∶100, BOSTER, Wuhan, China) overnight at 4°C.

Techniques: Incubation, Confocal Microscopy, Immunostaining, Expressing

Journal: PLoS ONE

Article Title: Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix

doi: 10.1371/journal.pone.0107339

Figure Lengend Snippet: NPWT-treated and static control group cell clots were harvested before and 1, 3, and 7 days after incubation (n = 3 per timepoint for each group) and analyzed by real-time RT-PCR. The expressions of the specific osteogenic genes (A) ALP, (B) OC, and (D) COL1 were higher in NPWT group than those in control group (* p <0.05, ** p <0.01). The NPWT group also expressed higher levels of the osteogenic transcription factor Cbfa1 (also known as Runx2) and the mechanosignaling molecule integrin β5 (ITGB5) (C) and (E) (* p <0.05, ** p <0.01).

Article Snippet: Then they were blocked with goat serum, and incubated with the specific rat primary antibodies against collagen type 1 (COL1) (1∶100, BOSTER, Wuhan, China) overnight at 4°C.

Techniques: Control, Incubation, Quantitative RT-PCR

Journal: Stem Cell Research & Therapy

Article Title: C/EBPα regulates the fate of bone marrow mesenchymal stem cells and steroid-induced avascular necrosis of the femoral head by targeting the PPARγ signalling pathway

doi: 10.1186/s13287-022-03027-3

Figure Lengend Snippet: Establishment and evaluation of SANFH rat models. a Scheme of animal treatments. b The coronal (COR), transverse (TRA) and sagittal (SAG) sections of the femoral head were reconstructed by the micro-CT images in the control and model groups. c Quantitative analysis of related parameters of micro-CT. d H&E staining of the femoral head after decalcification, with black arrows indicating trabecular bone, red arrows indicating fat vacuoles, and yellow arrows indicating empty bone lacunae. e, f IHC staining of COL1 in the femoral head and quantitative analysis showed the IOD in the control and model groups. g The expression levels of femoral head-related proteins in the control and model groups were detected by western blotting, and β-actin was used for normalization and quantitative analysis by ImageJ. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The remaining femoral head sections were dewaxed, antigen recovered, incubated with the primary antibody (rabbit polyclonal anti-type 1 collagen (COL1) and anti-PPARγ, Proteintech Group, Inc.), and then incubated with the appropriate horseradish peroxide-coupled secondary antibody.

Techniques: Micro-CT, Control, Staining, Immunohistochemistry, Expressing, Western Blot

Journal: BMC Urology

Article Title: Role of human adipose-derived stem cells (hADSC) on TGF-β1, type I collagen, and fibrosis degree in bladder obstruction model of Wistar rats

doi: 10.1186/s12894-022-01019-2

Figure Lengend Snippet: Effects of ADSC to collagen type I

Article Snippet: Prior to the procedure, the sample tissue was allowed to reach room temperature and then centrifuged at 2000–3000 RPM for 20 min. ELISA (enzyme-linked immunosorbent assay) measurements were performed to evaluate the TGF-1 and collagen type 1. (FineTest ® Rat Col1 ELISA kit range 0.156-10 ng/ml and FineTest ® TGF-1 ELISA kit range 31.25–2000 pg/ml).

Techniques: